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Adherent cells were scrapped, then centrifuged at 4C and 13000 g for 15 min, and the resulting supernatants processed using established protocols.[19] Intracellular levels of 1-MNA and as well as the IS were determined from LC/MS/MS peak areas

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Following these early observations, the up-regulation of plant GST genes has often been used as an indicator of oxidative stress and HR in plant-bacterium interactions (Alvarez et al., 2000)

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