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All yeast strains used in this study (Supplementary Table 7) were grown as previously described in Hartwells Complete (HC) medium supplemented with 2% (w/v) glucose as carbon source 79 and lacking histidine for plasmid selection unless stated otherwise

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In the Real-Time PCR Detection System, RT-PCR was carried out with 5 ng of cDNA per reaction using 25 L of SYBR Green QPCR Mix (Solis BioDyne) containing 20 M of specific primers

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