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The physical shearing force can tear apart the delicate amino acid chains

Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from the mouse kidney and HK-2 cells using a column-type animal tissue total RNA extraction and purification kit (Sangon Biotech Co., Ltd.), and then reverse transcribed to synthesize complementary DNA using a PrimeScript RT Reagent Kit with gDNA Eraser Kit (Takara Bio, Inc.)

Small-molecule neuroprotective compounds (J-147, 9-Me-BC): studied for mitochondrial and neurotrophic signalling outside the peptide class

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