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In Vitro 52 PinheiroJ.BatesD.DebRoyS.SarkarD.R Core Team (2009)

Reacting at room temperature for 3H, adding 10mL of glacial methyl tert-ether, precipitating a white solid, centrifuging, adding methyl tert-ether into the product, washing for 3 times, drying, and purifying by reversed-phase HPLC to obtain 90mg of semaglutide with the purity of 98.54%, wherein the liquid phase chromatogram is shown in figure 10, the mass chromatogram is shown in figure 11, and the molecular weight of the semaglutide is MS: M/z =4113.16 (M + H) + ) Example 10 Synthesis of semaglutide: 100mg of compound 4 obtained in example 6 and having a purity of about 76.13% was added to an iced freshly prepared 1mL lysate (trifluoroacetic acid: 1,2-ethanedithiol: triisopropylsilane = 95