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Briefly, GSTO1-1 pQE30 plasmid was transformed into M15 cells, which were grown in Luria-Bertani broth supplemented with 100 g ml 1 ampicillin and 25 g ml 1 kanamycin at 37 C to an OD 600 of 0.8 and induced with 0.1 mM isopropyl thio- -D-galactoside for 4 h

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Genome engineering with CRISPR/Cas9 has not only been used to introduce a knockout of the GLA gene, but also to correct gene mutations in patient-derived iPSCs [37, 74, 80, 86]