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Five unique GSTP1 -specific shRNA plasmids (clones TRCN0000083 773 , TRCN0000083 774 , TRCN0000083 775 , TRCN0000083 776 and TRCN0000083 777 ) and a negative control plasmid (empty vector control, SHC001) were purchased as glycerol stocks and plasmid DNA extracted using plasmid DNA maxi prep kits (Qiagen, Manchester, UK) according to the manufacturers instructions

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Weight loss can help mitigate the symptoms of PCOS, but it won't necessarily address the hormonal imbalances associated with the condition, which make it difficult to lose weight

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