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In addition, some known regulators of sporulation, such as the positive biofilm regulator SinI, 22 are upregulated, while the negative biofilm regulator PlcR, which is negatively controlled by Spo0A-P, 23 is downregulated

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It serves as a required substrate for enzymes such as sirtuins and poly(ADP-ribose) polymerases (PARPs). Sirtuins regulate cellular stress responses, metabolic homeostasis, and genomic stability, while PARPs play a central role in detecting DNA damage and initiating repair processes