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Elevated plasma level of visfatin/pre-B cell colony-enhancing factor in patients with type 2 diabetes mellitus

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Briefly, urine samples (500 l), HepaRG cell and hepatocyte samples (300 l), and microbiota samples (150 l) were diluted to 1 ml of HPLC grade water, acidified with 1% formic acid and extracted using Strata-X SPE