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Prolotherapy (short for "proliferation therapy") is a regenerative injection treatment that involves injecting an irritant solution into damaged or weakened ligaments, tendons, or joints

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Mild bloating or early satiety , likely related to delayed gastric emptying

The supernatant from the hypotonic solution was combined with the supernatant from the hypertonic solution, centrifuged to remove debris, and dialyzed against 20LmM Tris, pH 7.5 and 150LmM NaCl (Tris-buffered saline, TBS) overnight at 4 C