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HEK293T cells in 12 well plates were co-transfected for 24 hours with Lipofectamine 2000 using the following quantities of DNA: 0.5 g each of GLP-1R-SmBiT and LgBiT-mini-G, or 0.05 g each of GLP-1R-SmBiT and LgBiT-mini-G with 0.9 g empty vector DNA (pcDNA3.1)

Conversely, supplementing with omega-3 fatty acids may reduce levels of inflammation and has been found to reduce acne severity (36)

However, an imbalance between the two results in cellular degradation and the initiation of cell death mechanisms (Palikaras and Tavernarakis, 2014)