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Samples were then precipitated with 1 mL absolute MeOH at 40 C for 2 h, centrifuged (16,000 g ), and the obtained supernatants were evaporated, and the residue was resuspended and sonicated in 100 L 50 mM HEPES buffer (pH 7.4)

(2020) , in obese mice, found slight changes in specific gut bacteria Han et al

Int J Mol Sci (2021) 22(24):13455

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doi: 10.1172/JCI76348 67 SagonowskyE